Recording and controlling the 4D light field in a microscope using microlens arrays

@article{Levoy2009RecordingAC,
  title={Recording and controlling the 4D light field in a microscope using microlens arrays},
  author={M. Levoy and Z. Zhang and I. McDowall},
  journal={Journal of Microscopy},
  year={2009},
  volume={235}
}
By inserting a microlens array at the intermediate image plane of an optical microscope, one can record four‐dimensional light fields of biological specimens in a single snapshot. Unlike a conventional photograph, light fields permit manipulation of viewpoint and focus after the snapshot has been taken, subject to the resolution of the camera and the diffraction limit of the optical system. By inserting a second microlens array and video projector into the microscope's illumination path, one… Expand
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References

SHOWING 1-10 OF 65 REFERENCES
Light field microscopy
TLDR
A prototype light field microscope is demonstrated, its optical performance is analyzed, and perspective views, focal stacks, and reconstructed volumes are shown for a variety of biological specimens. Expand
An optical sectioning programmable array microscope implemented with a digital micromirror device
TLDR
The features of a PAM incorporating a digital micromirror device are reported, including the axial sectioning response to fluorescent thin films and the imaging of biological specimens, which demonstrated higher signal levels and dynamic range despite a shorter acquisition time. Expand
Light Field Photography with a Hand-held Plenoptic Camera
This paper presents a camera that samples the 4D light field on its sensor in a single photographic exposure. This is achieved by inserting a microlens array between the sensor and main lens,Expand
Optical recipes for light field microscopes
In our SIGGRAPH 2006 paper "Light Field Microscopy" [Levo y 2006], figure 2 gives the optical layout of our prototype. While this figure and its accompanying caption are not incorrect, they also doExpand
Digital correction of lens aberrations in light field photography
Digital light field photography consists of recording the radiance along all rays (the 4D light field) flowing into the image plane inside the camera, and using the computer to control the finalExpand
Polarized light field microscopy: an analytical method using a microlens array to simultaneously capture both conoscopic and orthoscopic views of birefringent objects
  • R. Oldenbourg
  • Materials Science, Medicine
  • Journal of microscopy
  • 2008
TLDR
The vastly improved throughput and quantitative analysis afforded by the light field LC‐PolScope make it the instrument of choice for measuring 3D birefringence parameters of complex structures. Expand
Dynamic contrast enhancement in widefield microscopy using projector-generated illumination patterns
We present a simple and cost-effective optical protocol to realize contrast-enhancement imaging (such as dark-field, optical-staining and oblique illumination microscopy) of transparent samples on aExpand
SLM Microscopy: Scanless Two-Photon Imaging and Photostimulation with Spatial Light Modulators
TLDR
A “scanless” microscope that uses a diffractive spatial light modulator (SLM) to shape an incoming two-photon laser beam into any arbitrary light pattern, which allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision. Expand
Synthetic aperture confocal imaging
TLDR
This paper adapts confocal imaging to large-scale scenes by replacing the optical apertures used in microscopy with arrays of real or virtual video projectors and cameras, and extracts mattes that can be used to selectively illuminate any plane in the scene. Expand
Programmable array microscopy with a ferroelectric liquid-crystal spatial light modulator.
TLDR
A programmable array microscope that uses a ferroelectric liquid-crystal spatial light modulator (SLM) for dynamic generation of scanning apertures and integration of the microscope output across all aperture frames produces a confocal image. Expand
...
1
2
3
4
5
...