Reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in Saccharomyces cerevisiae

@article{Rugbjerg2013ReconstructionOT,
  title={Reconstruction of the biosynthetic pathway for the core fungal polyketide scaffold rubrofusarin in Saccharomyces cerevisiae},
  author={Peter Rugbjerg and Michael Naesby and U. Mortensen and R. J. Frandsen},
  journal={Microbial Cell Factories},
  year={2013},
  volume={12},
  pages={31 - 31}
}
BackgroundFungal polyketides include commercially important pharmaceuticals and food additives, e.g. the cholesterol-lowering statins and the red and orange monascus pigments. Presently, production relies on isolation of the compounds from the natural producers, and systems for heterologous production in easily fermentable and genetically engineerable organisms, such as Saccharomyces cerevisiae and Escherichia coli are desirable. Rubrofusarin is an orange polyketide pigment that is a common… Expand
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References

SHOWING 1-10 OF 40 REFERENCES
Production of a polyketide natural product in nonpolyketide-producing prokaryotic and eukaryotic hosts.
TLDR
The heterologous expression systems described will facilitate the manipulation of PKS genes and consequent production of novel engineered polyketides and polyketide libraries. Expand
Optimization of heterologous production of the polyketide 6‐MSA in Saccharomyces cerevisiae
TLDR
This study demonstrates the heterologous expression of 6‐methylsalicylic acid synthase (6‐MSAS), naturally produced by Penicillium patulum, in the yeast Saccharomyces cerevisiae, and co‐expressed PPTases encoded by either sfp from Bacillus subtilis or by npgA from Aspergillus nidulans. Expand
Establishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics
TLDR
This work has developed a streamlined method for isolating biosynthetic genes from fungal sources and producing bioactive molecules in an engineered Saccharomyces cerevisiae host strain using overlap extension PCR and yeast homologous recombination. Expand
Insights into Radicicol Biosynthesis via Heterologous Synthesis of Intermediates and Analogs*
TLDR
The complete reconstitution of Rdc5 and Rdc1 activities both in vitro and in Saccharomyces cerevisiae uncovered the earliest resorcylic acid lactone intermediate of the radicicol biosynthetic pathway, (R)-monocillin II. Expand
Characterization of a polyketide synthase in Aspergillus niger whose product is a precursor for both dihydroxynaphthalene (DHN) melanin and naphtho-γ-pyrone.
TLDR
AlbA is a predicted ortholog of alb1 from Aspergillus fumigatus which is responsible for production of the naphtho-γ-pyrone precursor for the 1,8-dihydroxynaphthalene (DHN) melanin/spore pigment. Expand
The biosynthetic pathway for aurofusarin in Fusarium graminearum reveals a close link between the naphthoquinones and naphthopyrones
TLDR
Replacement of aurR1 with hygB shows that it encodes a positively acting transcription factor that is required for the full expression of PKS12, aurJ, aurF, gip1 and FG02329.1, which belong to the gene cluster, and bio‐ and chemoinformatics combined with chemical analysis of replacement mutants indicate a five‐step enzyme catalysed pathway for the biosynthesis of aurofusarin. Expand
Putative Polyketide Synthase and Laccase Genes for Biosynthesis of Aurofusarin in Gibberella zeae
TLDR
Targeted gene deletion and complementation analyses confirmed that both Gip1 and PKS12 are required for aurofusarin production in G. zeae and could help in studies of their toxicity in domesticated animals. Expand
Metabolic Engineering of Saccharomyces cerevisiae for Astaxanthin Production and Oxidative Stress Tolerance
TLDR
Interestingly, these ketocarotenoids conferred oxidative stress tolerance on S. cerevisiae cells and has potential for overproduction of astaxanthin and breeding of novel oxidative stress-tolerant yeast strains. Expand
Production of the polyketide 6-MSA in yeast engineered for increased malonyl-CoA supply.
TLDR
In order to improve the production of 6-MSA by enhancing the supply of precursors, the promoter of the gene (ACC1) encoding acetyl-CoA carboxylase was replaced with a strong, constitutive promoter (TEF1p) in a strain harboring two plasmids carrying the genes encoding 6-MSAS. Expand
Two Novel Classes of Enzymes Are Required for the Biosynthesis of Aurofusarin in Fusarium graminearum*
TLDR
Functional information is provided on two novel classes of proteins and their contribution to polyketide pigment biosynthesis in Fusarium graminearum and aurZ and aurS. Expand
...
1
2
3
4
...