Reconstruction of neural tube-like structures in vitro from primary neural precursor cells.

  title={Reconstruction of neural tube-like structures in vitro from primary neural precursor cells.},
  author={Yasuhiro Tomooka and Hiroshi Kitani and Naihe Jing and Michiya Matsushima and Teruyo Sakakura},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={90 20},
Vertebrate central nervous system develops from a neural tube derived from the embryonic ectoderm. In mouse, the neural tube around embryonic day 10 primarily consists of neural precursor cells (NPCs). During the development of embryonic central nervous system, NPCs proliferate and migrate outward; thus later stages show NPCs toward the lumen of the neural tube and neurofilament-positive differentiated cells toward the periphery. In conventional liquid culture, NPCs isolated from mouse on… CONTINUE READING
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