Reconstruction of an enzyme by domain substitution effectively switches substrate specificity

@article{Houghton1989ReconstructionOA,
  title={Reconstruction of an enzyme by domain substitution effectively switches substrate specificity},
  author={John Edgar Houghton and G A O'donovan and James R. Wild},
  journal={Nature},
  year={1989},
  volume={338},
  pages={172-174}
}
THE polar domains of the two transcarbamoylases, aspartate transcarbamoylase (ATCase) and ornithine transcarbamoylase, (OTCase) from Escherichia coli bind the common substrate carbamoyl phosphate and share extensive amino-acid sequence homology1,2. The equatorial domains of the two enzymes differ in their substrate specificity (ATCase binds aspartate, OTCase binds ornithine) and have decreased sequence identity. While addressing the conservation of specific protein interactions during the… CONTINUE READING