Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

  title={Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy},
  author={Philipp J. Keller and Annette D. Schmidt and Joachim Wittbrodt and Ernst H. K. Stelzer},
  pages={1065 - 1069}
A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. [] Key Method We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of…

The zebrafish digital embryo: in toto reconstruction of zebrafish early embryonic development with digital scanned laser light sheet fluorescence microscopy

Using DSLM, the development of entire zebrafish embryos in vivo and with sub-cellular resolution is recorded and defects in embryonic development or disease models can now be analyzed and understood on a quantitative level.

Methodology for Reconstructing Early Zebrafish Development From In Vivo Multiphoton Microscopy

This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution.

3D Light-Sheet Fluorescence Microscopy of Cranial Neurons and Vasculature during Zebrafish Embryogenesis

This work uses light-sheet fluorescence microscopy to image the entire head of live transgenic zebrafish embryos and simultaneously imaged cranial neurons and blood vessels during embryogenesis, generating comprehensive 3D maps that provide insight into the coordinated morphogenesis of the nervous system and vasculature during early development.

Digital scanned laser light-sheet fluorescence microscopy (DSLM) of zebrafish and Drosophila embryonic development.

A sample preparation and imaging protocol for studying the development of whole zebrafish and Drosophila embryos using digital scanned laser light-sheet fluorescence microscopy, which provides up to 50 times faster imaging speeds and a 10-100-fold higher signal-to-noise ratio.

Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy

A framework for imaging and reconstructing unstained whole zebrafish embryos for their first 10 cell division cycles is designed and measurements along the cell lineage are reported with micrometer spatial resolution and minute temporal accuracy.

Light sheet microscopy for real-time developmental biology.

Towards comprehensive cell lineage reconstructions in complex organisms using light‐sheet microscopy

It is argued that comprehensive cell lineage reconstructions are finally within reach for many key model organisms, including fruit fly, zebrafish and mouse.

Introduction Live Imaging of Mouse Embryos

The development of ex vivo embryo culture methods combined with high-resolution imaging now provides a strong platform for observing morphogenetic events as they occur within the developing embryo, and the advantages of live embryo imaging for observing dynamic morphogenic events in vivo are discussed.

Live Imaging of Whole Mouse Embryos during Gastrulation: Migration Analyses of Epiblast and Mesodermal Cells

Deep and high time-resolution images of GFP-expressing nuclei and following 3D tracking analysis revealed the following findings: Interkinetic nuclear migration (INM) occurs in the epiblast at embryonic day (E)6 and 6.5 and Mesodermal cells migrate not as a sheet but as individual cells without coordination.



Analysis of Cell Movements in Zebrafish Embryos

This chapter offers a conceptual background for analysis of gastrulation cell movements by reviewing how region specific cell movements shape the wild-type zebra fish embryo, and how defective morphogenetic movements alone or in combination with altered cell fate specification distort the body plans of known zebrafish mutants.

Oriented cell divisions and cellular morphogenesis in the zebrafish gastrula and neurula: a time-lapse analysis.

It is found that the onset of gastrulation is accompanied by major changes in cell behaviour, and ML divisions within the neuroepithelium appear to be better correlated with changes in tissue morphology associated with neurulation.

Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy

Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination

Distribution of tissue progenitors within the shield region of the zebrafish gastrula.

The data show that progenitor cells of the neural, notochordal, somitic and endodermal lineages were all present within the embryonic shield region, and that these progenitors were arranged as intermingled populations.

Single cell lineage and regionalization of cell populations during Medaka neurulation

Continuous live recording of labeled single precursor cells and computer graphics-assisted data analysis, which are presented for the first time in this study, provide excellent means with which to analyze essential cellular processes in organogenesis.

Initiation of convergence and extension movements of lateral mesoderm during zebrafish gastrulation

Using mathematical modeling, it is demonstrated that directional preference is sufficient to account for mesoderm convergence and extension, and that, at minimum, two sources of guidance cues could orient cell paths realistically if located in the dorsal midline.

Individual Cell Migration Serves as the Driving Force for Optic Vesicle Evagination

The analysis of mutants demonstrated that the retina-specific transcription factor rx3 determines the convergence and migration behaviors of RPCs, and hence, the migration of individual cells mediates essential steps of organ morphogenesis.

Dorso-ventral polarity of the zebrafish embryo is distinguishable prior to the onset of gastrulation

The embryonic dorso-ventral polarity axis is morphologically distinguishable prior to the onset of gastrulation; and the involution of deep layer cells starts on the prospective dorsal side of the embryo.