Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

@article{Keller2008ReconstructionOZ,
  title={Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy},
  author={P. J. Keller and A. Schmidt and J. Wittbrodt and E. Stelzer},
  journal={Science},
  year={2008},
  volume={322},
  pages={1065 - 1069}
}
  • P. J. Keller, A. Schmidt, +1 author E. Stelzer
  • Published 2008
  • Medicine, Biology
  • Science
  • A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. [...] Key Method We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of…Expand Abstract

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