Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy

@article{Keller2008ReconstructionOZ,
  title={Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy},
  author={Philipp J. Keller and Annette D. Schmidt and Joachim Wittbrodt and Ernst H. K. Stelzer},
  journal={Science},
  year={2008},
  volume={322},
  pages={1065 - 1069}
}
A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. [] Key Method We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos," that is, comprehensive databases of cell positions, divisions, and migratory tracks. Our analysis of…

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Live Imaging of Whole Mouse Embryos during Gastrulation: Migration Analyses of Epiblast and Mesodermal Cells

TLDR
Deep and high time-resolution images of GFP-expressing nuclei and following 3D tracking analysis revealed the following findings: Interkinetic nuclear migration (INM) occurs in the epiblast at embryonic day (E)6 and 6.5 and Mesodermal cells migrate not as a sheet but as individual cells without coordination.
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