Reconstitution of water channel function of aquaporins 1 and 2 by expression in yeast secretory vesicles.

@article{Coury1998ReconstitutionOW,
  title={Reconstitution of water channel function of aquaporins 1 and 2 by expression in yeast secretory vesicles.},
  author={Larry A Coury and John C. Mathai and G V Ramesh Prasad and Jeffrey L Brodsky and Peter Agre and Mark Lawrence Zeidel},
  journal={American journal of physiology. Renal physiology},
  year={1998},
  volume={274 1},
  pages={F34-F42}
}
Aquaporins 1 (AQP1) and 2 (AQP2) were expressed in the yeast secretory mutant sec6-4. The mutant accumulates post-Golgi, plasma membrane-targeted vesicles and may be used to produce large quantities of membrane proteins. AQP1 or AQP2 were inducibly expressed in yeast and were localized within isolated sec6-4 vesicles by immunoblot analysis. Secretory vesicles containing AQP1 and AQP2 exhibited high water permeabilities and low activation energies for water flow, indicating expression of… CONTINUE READING