Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein

@article{Betts1994ReconstitutionOT,
  title={Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein},
  author={Scott D. Betts and Todd M. Hachigian and Eran Pichersky and Charles F. Yocum},
  journal={Plant Molecular Biology},
  year={1994},
  volume={26},
  pages={117-130}
}
The psbO gene of cyanobacteria, green algae and higher plants encodes the precursor of the 33 kDa manganese-stabilizing protein (MSP), a water-soluble subunit of photosystem II (PSII). Using a pET-T7 cloning/expression system, we have expressed in Escherichia coli a full-length cDNA clone of psbO from Arabidopsis thaliana. Upon induction, high levels of the precursor protein accumulated in cells grown with vigorous aeration. In cells grown under weak aeration, the mature protein accumulated… CONTINUE READING
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