Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein

  title={Reconstitution of the spinach oxygen-evolving complex with recombinant Arabidopsis manganese-stabilizing protein},
  author={Scott D. Betts and Todd M. Hachigian and Eran Pichersky and Charles F. Yocum},
  journal={Plant Molecular Biology},
The psbO gene of cyanobacteria, green algae and higher plants encodes the precursor of the 33 kDa manganese-stabilizing protein (MSP), a water-soluble subunit of photosystem II (PSII). Using a pET-T7 cloning/expression system, we have expressed in Escherichia coli a full-length cDNA clone of psbO from Arabidopsis thaliana. Upon induction, high levels of the precursor protein accumulated in cells grown with vigorous aeration. In cells grown under weak aeration, the mature protein accumulated… CONTINUE READING
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Effect of the 33-kDa protein on the S-state transitions in photosynthetic oxygen evolution

  • M Miyao, N Murata, J Lavorel, B Maison-Peteri, A Boussac, A-L Etienne
  • Biochim Biophys Acta 890:151-159
  • 1987
Highly Influential
4 Excerpts

Calcium reconstitutes high rates of oxygen evolution in polypeptide depleted photosystem II preparations

  • DF Ghanotakis, GT Babcock, CF Yocum
  • FEBS Lett 167: 127-130
  • 1984
Highly Influential
4 Excerpts

Deletion of the gene encoding the photosystem II 33 kDa protein from Synechocystis PCC 6803 does not inactivate water-splitting but increases vulnerability to photoinhibition

  • SR Mayes, KM Cook, SJ Self, Z Zhang, J Barber
  • Biochim Biophys Acta 1060:1-12
  • 1991

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