Reconstitution of the ribonucleotide reductase enzyme from Ehrlich tumor cells.

@article{Cory1978ReconstitutionOT,
  title={Reconstitution of the ribonucleotide reductase enzyme from Ehrlich tumor cells.},
  author={Joseph G. Cory and A E Fleischer and J B Munro},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 9},
  pages={2898-901}
}
Ribonucleotide reductase from Ehrlich tumor cells was separated by chromatography on blue dextran/Sepharose into two protein fractions (Tris and Dye fractions). Neither fraction alone had reductase activity, but when combined, constituted an active enzyme system. Heat treatment of either fraction resulted in an inactive combination. The approximate molecular size of the active component of the Tris and Dye fractions was determined to be 5.7 S and 6.5 S, respectively, compared to 9 S for the… CONTINUE READING