Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins.

@article{Tienson2009ReconstitutionOT,
  title={Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins.},
  author={H. Tienson and Deepa V. Dabir and Sonya E. Neal and R. Loo and Samuel A Hasson and P. Boontheung and Sung-Kun Kim and J. Loo and C. Koehler},
  journal={Molecular biology of the cell},
  year={2009},
  volume={20 15},
  pages={
          3481-90
        }
}
Mia40 and Erv1 execute a disulfide relay to import the small Tim proteins into the mitochondrial intermembrane space. Here, we have reconstituted the oxidative folding pathway in vitro with Tim13 as a substrate and determined the midpoint potentials of Mia40 and Tim13. Specifically, Mia40 served as a direct oxidant of Tim13, and Erv1 was required to reoxidize Mia40. During oxidation, four electrons were transferred from Tim13 with the insertion of two disulfide bonds in succession. The extent… Expand
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Redox Characterization of Proteins Involved in the Mitochondrial Intermembrane Space Pathway
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References

SHOWING 1-10 OF 54 REFERENCES
The Erv1-Mia40 disulfide relay system in the intermembrane space of mitochondria.
  • K. Hell
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 2008
The role of the Tim8p–Tim13p complex in a conserved import pathway for mitochondrial polytopic inner membrane proteins
Oxidative folding competes with mitochondrial import of the small Tim proteins.
Zinc binding stabilizes mitochondrial Tim10 in a reduced and import-competent state kinetically.
The essential mitochondrial protein Erv1 cooperates with Mia40 in biogenesis of intermembrane space proteins.
A role for cytochrome c and cytochrome c peroxidase in electron shuttling from Erv1
A Disulfide Relay System in the Intermembrane Space of Mitochondria that Mediates Protein Import
Mia40, a novel factor for protein import into the intermembrane space of mitochondria is able to bind metal ions
...
1
2
3
4
5
...