Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins.

@article{Tienson2009ReconstitutionOT,
  title={Reconstitution of the mia40-erv1 oxidative folding pathway for the small tim proteins.},
  author={Heather L. Tienson and Deepa V. Dabir and Sonya E. Neal and Rachel Loo and Samuel A Hasson and Pinmanee Boontheung and Sung-Kun Kim and Joseph A Loo and Carla M Koehler},
  journal={Molecular biology of the cell},
  year={2009},
  volume={20 15},
  pages={
          3481-90
        }
}
Mia40 and Erv1 execute a disulfide relay to import the small Tim proteins into the mitochondrial intermembrane space. Here, we have reconstituted the oxidative folding pathway in vitro with Tim13 as a substrate and determined the midpoint potentials of Mia40 and Tim13. Specifically, Mia40 served as a direct oxidant of Tim13, and Erv1 was required to reoxidize Mia40. During oxidation, four electrons were transferred from Tim13 with the insertion of two disulfide bonds in succession. The extent… CONTINUE READING

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