Reconstitution of the lysosomal proton pump.

  title={Reconstitution of the lysosomal proton pump.},
  author={M D'souza and Suresh V Ambudkar and J. Thomas August and Peter Maloney},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={84 20},
Lysosomal membrane proteins solubilized with octyl beta-D-glucopyranoside were reconstituted into proteoliposomes using acetone/ether-washed phospholipids from Escherichia coli. Assays of the quenching of acridine orange fluorescence showed that addition of both ATP and valinomycin to K+-loaded proteoliposomes led to the formation of a pH gradient that was acidic inside. ATP-driven acidification took place in the absence of permeant anions and was inhibited by the "protonophore… CONTINUE READING