Reconstitution of the hexose phosphate translocator from the envelope membranes of wheat endosperm amyloplasts.

@article{Tetlow1996ReconstitutionOT,
  title={Reconstitution of the hexose phosphate translocator from the envelope membranes of wheat endosperm amyloplasts.},
  author={Ian J Tetlow and Caroline G. Bowsher and Michael J. Emes},
  journal={The Biochemical journal},
  year={1996},
  volume={319 ( Pt 3)},
  pages={717-23}
}
Amyloplasts were isolated and purified from wheat endosperm and the envelope membranes reconstituted into liposomes. Envelope membranes were solubilized in n-octyl beta-D-glucopyranoside and mixed with liposomes supplemented with 5.6 mol% cholesterol to produce proteoliposomes of defined size, which showed negligible leakage of internal substrates. Transport experiments with proteoliposomes revealed a counter-exchange of glucose 1-phosphate (Glc1P), glucose 6-phosphate (Glc6P), inorganic… CONTINUE READING