Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase

@inproceedings{Beckwith2013ReconstitutionOT,
  title={Reconstitution of the 26S proteasome reveals functional asymmetries in its AAA+ unfoldase},
  author={Robyn Beckwith and E. Estrin and Evan J. Worden and Andreas Martin},
  booktitle={Nature Structural &Molecular Biology},
  year={2013}
}
The 26S proteasome is the major eukaryotic ATP-dependent protease, yet the detailed mechanisms used by the proteasomal heterohexameric AAA+ unfoldase to drive substrate degradation remain poorly understood. To perform systematic mutational analyses of individual ATPase subunits, we heterologously expressed the unfoldase subcomplex from Saccharomyces… CONTINUE READING