Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains.

  title={Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains.},
  author={Ikuro Nitta and Yasuhiro Kamada and H Noda and Tadashi Ueda and Kunihiko Watanabe},
  volume={281 5377},
It was recently demonstrated that peptide bond formation can occur using an Escherichia coli naked 23S ribosomal RNA without any of the ribosomal proteins. Here, the six domains of the 23S ribosomal RNA were individually synthesized and shown to be capable, when complexed together, of stimulating the reaction. Omission and addition experiments indicated that the activity could be reconstituted solely by domain V at a concentration 10 times higher than that of the intact 23S ribosomal RNA… CONTINUE READING

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