Reconstitution of mitochondrial presequence translocase into proteoliposomes.

Abstract

The isolation and functional reconstitution of large membrane protein complexes is an important step towards the biochemical characterization of such sophisticated molecular machines. Reconstitution is a multistep process that requires the mild solubilization of membrane protein complexes from native membrane preparations, the purification of the complexes… (More)
DOI: 10.1007/978-1-62703-487-6_21

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