Reconstitution of human base excision repair with purified proteins.

  title={Reconstitution of human base excision repair with purified proteins.},
  author={Iain D Nicholl and K Nealon and Mark K. Kenny},
  volume={36 24},
Base excision repair is a major mechanism for correcting aberrant DNA bases. We are using an in vitro base excision repair assay to fractionate and purify proteins from a human cell extract that are involved in this type of repair. Three fractions are required to reconstitute base excision repair synthesis using a uracil-containing DNA as a model substrate. We previously showed that one fraction corresponds to DNA polymerase beta. A second fraction was extensively purified and found to possess… CONTINUE READING

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