Reconstitution of damage DNA excision reaction from SV40 minichromosomes with purified nucleotide excision repair proteins.

@article{Araki2000ReconstitutionOD,
  title={Reconstitution of damage DNA excision reaction from SV40 minichromosomes with purified nucleotide excision repair proteins.},
  author={Marito Araki and Chikahide Masutani and Toshio Maekawa and Yoshinori Watanabe and Ayumi Yamada and Rika Kusumoto and Dennis Sakai and Kaoru Sugasawa and Yoshiaki Ohkuma and Fumio Hanaoka},
  journal={Mutation research},
  year={2000},
  volume={459 2},
  pages={147-60}
}
We previously constructed the cell-free nucleotide excision repair (NER) assay system with UV-irradiated SV40 minichromosomes to analyze the mechanism of NER reaction on chromatin DNA. Here we investigate the factor that acts especially on nucleosomal DNA during the damage excision reaction, and reconstitute the damage excision reaction on SV40 minichromosomes. NER-proficient HeLa whole cell extracts were fractionated, and the amounts of known NER factors involved in the column fractions were… CONTINUE READING