Reconstitution of carbamylcholine-dependent sodium ion flux and desensitization of the acetylcholine receptor from Torpedo californica.

@article{Epstein1978ReconstitutionOC,
  title={Reconstitution of carbamylcholine-dependent sodium ion flux and desensitization of the acetylcholine receptor from Torpedo californica.},
  author={Murray Epstein and Efraim Racker},
  journal={The Journal of biological chemistry},
  year={1978},
  volume={253 19},
  pages={6660-2}
}
Membranes rich in acetylcholine receptor were isolated from Torpedo californica by a modification of the procedure of Sobel et al. (Sobel, A., Weber, M., and Changeux, J.-P. (1977) Eur. J. Biochem. 80, 215-224). The receptor was extracted with 2% potassium cholate in the presence of 2.5% soybean phospholipids. After reconstitution by the cholate dialysis procedure, the vesicles exhibited a rapid, carbamylcholine-dependent uptake of 22Na+, which was inhibited by alpha-bungarotoxin and several… CONTINUE READING
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Receptors and Reconstitution (Cuatrecaaaa

  • M. E. Eldefrawl, A. T. Eldefrawi
  • 1977

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