Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae.

  title={Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae.},
  author={Koichi Tagami and Chengwei Liu and Atsushi Minami and Motoyoshi Noike and Tetsuya Isaka and Shuhei Fueki and Yoshihiro Shichijo and Hiroaki Toshima and Katsuya Gomi and Tohru Dairi and Hideaki Oikawa},
  journal={Journal of the American Chemical Society},
  volume={135 4},
Indole-diterpenes represented by paxilline share a common pentacyclic core skeleton derived from indole and geranylgeranyl diphosphate. To shed light on the detailed biosynthetic mechanism of the paspaline-type hexacyclic skeleton, we examined the reconstitution of paxilline biosynthetic machinery in Aspergillus oryzae NSAR1. Stepwise introduction of the six pax genes enabled us to isolate all biosynthetic intermediates and to synthesize paxilline. In vitro and in vivo studies on the key… CONTINUE READING
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