Reconstitution of a chloride-translocating ATPase from Aplysia californica gut.

Abstract

Basolateral membranes of Aplysia foregut epithelia contain both a Cl(-)-stimulated ATPase activity and an ATP-dependent Cl- transport. The protein responsible for both of these biochemical activities (Cl- pump) can be solubilized and reconstituted into liposomes with the aid of the detergent digitonin. Proteoliposomal Cl- pump activity was inhibited by… (More)

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