Reconstitution of Src-dependent phospholipase Cgamma phosphorylation and transient calcium release by using membrane rafts and cell-free extracts from Xenopus eggs.

@article{Sato2003ReconstitutionOS,
  title={Reconstitution of Src-dependent phospholipase Cgamma phosphorylation and transient calcium release by using membrane rafts and cell-free extracts from Xenopus eggs.},
  author={Ken-ichi Sato and Alexander A. Tokmakov and Chang-li He and Manabu Kurokawa and Tetsushi Iwasaki and Mikako Shirouzu and Rafael A. Fissore and Shigeyuki Yokoyama and Yasuo Fukami},
  journal={The Journal of biological chemistry},
  year={2003},
  volume={278 40},
  pages={38413-20}
}
We reported previously that egg membrane rafts serve as a subcellular microdomain for sperm-dependent tyrosine kinase signaling in Xenopus fertilization. Moreover, we demonstrated that raft-associated Src tyrosine kinase was activated by sperm in vitro. Here we show that egg rafts incubated with sperm or hydrogen peroxide (H2O2) can promote Src-dependent phosphorylation of phospholipase Cgamma (PLCgamma) and transient calcium release in the extracts of unfertilized Xenopus eggs. In vivo egg… CONTINUE READING
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