Reconstitution of Na+/K+-ATPase into phosphatidylcholine vesicles by dialysis of nonionic alkyl maltoside detergents.

@article{Alpes1988ReconstitutionON,
  title={Reconstitution of Na+/K+-ATPase into phosphatidylcholine vesicles by dialysis of nonionic alkyl maltoside detergents.},
  author={H. Alpes and Hans-J{\"u}rgen Apell and Gunter Knoll and Helmut Plattner and Roland Riek},
  journal={Biochimica et biophysica acta},
  year={1988},
  volume={946 2},
  pages={
          379-88
        }
}
The reconstitution of Na+/K+-ATPase from outer medulla of rabbit kidney into large unilamellar liposomes was achieved through detergent removal by dialysis of mixed micellar solutions of synthetic dioleoyl phosphatidylcholine/octyl glucoside and Na+/K+-ATPase/decyl maltoside or decenyl maltoside. Tight, transport-active liposomes were formed when the lipid and the enzyme were solubilized separately in the nonionic detergents and mixed immediately before starting the dialysis. The two maltoside… CONTINUE READING
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