Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast.

@article{Kapoor2001ReconstitutionOE,
  title={Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast.},
  author={Priya Kapoor and Kathy Shire and Lori Frappier},
  journal={The EMBO journal},
  year={2001},
  volume={20 1-2},
  pages={222-30}
}
The EBNA1 protein of Epstein-Barr virus (EBV) mediates the partitioning of EBV episomes and EBV-based plasmids during cell division by a mechanism that appears to involve binding to the cellular EBP2 protein on human chromosomes. We have investigated the ability of EBNA1 and the EBV segregation element (FR) to mediate plasmid partitioning in Saccharomyces cerevisiae. EBNA1 expression alone did not enable the stable segregation of FR-containing plasmids in yeast, but segregation was rescued by… CONTINUE READING