Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities

@inproceedings{Sebesta2011ReconstitutionOD,
  title={Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities},
  author={Marek Sebesta and Peter Burkovics and Lajos Haracska and Lumir Krejci},
  booktitle={DNA repair},
  year={2011}
}
The error-free repair of double-strand DNA breaks by homologous recombination (HR) ensures genomic stability using undamaged homologous sequence to copy genetic information. While some of the aspects of the initial steps of HR are understood, the molecular mechanisms underlying events downstream of the D-loop formation remain unclear. Therefore, we have reconstituted D-loop-based in vitro recombination-associated DNA repair synthesis assay and tested the efficacy of polymerases Pol δ and Pol… CONTINUE READING

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