Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System

@article{Mueller1962ReconstitutionOC,
  title={Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System},
  author={Paul Mueller and Donald O. Rudin and H. Ti Tien and William C. Wescott},
  journal={Nature},
  year={1962},
  volume={194},
  pages={979-980}
}
THE formation of single, stable bimolecular lipid and proteolipid1 membranes up to 10 mm.2 in area has been accomplished routinely in 0.1 M saline solution by methods analogous to the formation of Hooke-Newton ‘secondary black’ in air soap films2–4. By forming such a membrane between two compartments filled with saline its transverse electrical properties can be measured, and controlled chemical investigations can be undertaken. 

Topics from this paper

Production of Isolated Giant Unilamellar Vesicles under High Salt Concentrations
TLDR
An overview of the existing methods for GUV production is given and efforts on forming single, free floating vesicles up to several tens of μm in diameter and at high yield in various buffer solutions with physiological ionic strength and pH are presented. Expand
Chemically driven phase separation in black lipid membranes and its coupling to membrane functions
Abstract We analysed the single-channel current fluctuations of gramicidin in bimolecular lipid membranes in order to demonstrate (i) the influence of protein binding to the lateral organisation of aExpand
Functional reassembly of membrane proteins in planar lipid bilayers.
TLDR
This work has shown that the reconstitution approach has proved to be valuable in efforts to understand the molecular mechanisms of membrane transport and energy transduction. Expand
Reconstitution of proteins on electroformed giant unilamellar vesicles.
In vitro reconstitution of simplified biological systems from molecular parts has proven to be a powerful method for investigating the biochemical and biophysical principles underlying cellularExpand
Trends in the development and application of functional biomembrane surfaces.
TLDR
The principal role of lipid biomembrane involves maintaining a concentration gradient of compounds inside the cells of living organisms, which are different from those outside together with maintaining an electric potential difference between the interior of the cell and the external fluid. Expand
Functional reconstitution of membrane proteins in planar lipid bilayer membranes
Understanding the mechanism of action of membrane proteins depends upon the convergence of knowledge about the structure of the protein with a detailed characterization of its function at theExpand
Structural Rearrangements in Lipid Bilayer Membranes
We will examine local structural rearrangements in lipid bilayers, which are of great functional significance for cell biology and biotechnology. The choice of this topic will permit interestingExpand
Induced excitability in reconstituted cell membrane structure.
TLDR
The kinetic and steady state aspects of these resistance changes are presented, and a theory is developed which accounts for the observed phenomena. Expand
Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay
  • A. Darszon
  • Biology, Medicine
  • Journal of bioenergetics and biomembranes
  • 1983
TLDR
The various strategies available today to reassemble functional membrane proteins into model lipid bilayer systems are described and the intimate relationships and complementarity between proteoliposomes and planar bilayers are illustrated. Expand
Generation of large and active membrane layers from thylakoid vesicles
Biological membranes are interfaces which separate an inner from an outer space. Analysis of the vectorial molecular mechanisms at work in such ‘in/out’ systems are difficult because those reactionsExpand
...
1
2
3
4
5
...

References

SHOWING 1-5 OF 5 REFERENCES
THE EFFECTS OF CURRENT FLOW ON BIOELECTRIC POTENTIAL : I. VALONIA
The effect of direct current flow upon the potential difference across the protoplasm of impaled Valonia cells was studied. Current density and direction were controlled in a bridge which balancedExpand
PROLONGED ACTION POTENTIALS FROM SINGLE NODES OF RANVIER
  • P. Mueller
  • Medicine, Chemistry
  • The Journal of general physiology
  • 1958
TLDR
One type of prolonged action potential can be observed in solutions which contain K or Rb ions at concentrations between 50 mM and 2 M and requires no K or Na ions in the external medium. Expand
Electrical Excitability of Isolated Frog Skin
IN the course of investigating the electrical properties of frog skin with short current pulses, it was discovered that this preparation is capable of exhibiting electrical excitability. The presentExpand
Proteolipides, a new type of tissue lipoproteins; their isolation from brain.
La stratification des lames liquides