Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System

@article{Mueller1962ReconstitutionOC,
  title={Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System},
  author={Paul Mueller and Donald O. Rudin and H. Ti Tien and William C. Wescott},
  journal={Nature},
  year={1962},
  volume={194},
  pages={979-980}
}
THE formation of single, stable bimolecular lipid and proteolipid1 membranes up to 10 mm.2 in area has been accomplished routinely in 0.1 M saline solution by methods analogous to the formation of Hooke-Newton ‘secondary black’ in air soap films2–4. By forming such a membrane between two compartments filled with saline its transverse electrical properties can be measured, and controlled chemical investigations can be undertaken. 

Production of Isolated Giant Unilamellar Vesicles under High Salt Concentrations

An overview of the existing methods for GUV production is given and efforts on forming single, free floating vesicles up to several tens of μm in diameter and at high yield in various buffer solutions with physiological ionic strength and pH are presented.

Functional reconstitution of membrane proteins in planar lipid bilayer membranes

Understanding the mechanism of action of membrane proteins depends upon the convergence of knowledge about the structure of the protein with a detailed characterization of its function at the

Structural Rearrangements in Lipid Bilayer Membranes

This work will examine local structural rearrangements in lipid bilayers, which are of great functional significance for cell biology and biotechnology and will permit interesting analogies to be drawn with classical electrochemistry and chemical kinetics.

Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay

  • A. Darszon
  • Biology
    Journal of bioenergetics and biomembranes
  • 1983
The various strategies available today to reassemble functional membrane proteins into model lipid bilayer systems are described and the intimate relationships and complementarity between proteoliposomes and planar bilayers are illustrated.

Composite S-layer lipid structures.

...

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