Reconstitution of B cell antigen receptor-induced signaling events in a nonlymphoid cell line by expressing the Syk protein-tyrosine kinase.

@article{Richards1996ReconstitutionOB,
  title={Reconstitution of B cell antigen receptor-induced signaling events in a nonlymphoid cell line by expressing the Syk protein-tyrosine kinase.},
  author={James D. Richards and Michael R Gold and S L Hourihane and Anthony L DeFranco and Linda Matsuuchi},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 11},
  pages={6458-66}
}
B cell antigen receptor (BCR) cross-linking activates both Src family and Syk tyrosine kinases, resulting in increased cellular protein-tyrosine phosphorylation and activation of several downstream signaling enzymes. To define the role of Syk in these events, we expressed the BCR in the AtT20 mouse pituitary cell line. These nonlymphoid cells endogenously expressed the Src family kinase Fyn but not Syk. Anti-IgM stimulation of these cells failed to induce most of the signaling events that occur… CONTINUE READING