Reconstitution in vitro of the pH-dependent aggregation of pancreatic zymogens en route to the secretory granule: implication of GP-2.

@article{Leblond1993ReconstitutionIV,
  title={Reconstitution in vitro of the pH-dependent aggregation of pancreatic zymogens en route to the secretory granule: implication of GP-2.},
  author={François A. Leblond and Gilles Viau and Jean Lain{\'e} and Denis Lebel},
  journal={The Biochemical journal},
  year={1993},
  volume={291 ( Pt 1)},
  pages={289-96}
}
Regulated secretory proteins are thought to be sorted in the trans-Golgi network (TGN) via selective aggregation. To elucidate the biogenesis of the secretory granule in the exocrine pancreas, we reconstituted in vitro the conditions of pH and ions believed to exist in the TGN using the end product of this sorting process, the zymogen granule contents. Protein aggregation was dependent on pH (acidic) and on the presence of cations (10 mM Ca2+, 150 mM K+) to reproduce the pattern of proteins… CONTINUE READING

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