Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans.

@article{Saiki2001ReconstitutionAP,
  title={Reconstitution and purification of cytolethal distending toxin of Actinobacillus actinomycetemcomitans.},
  author={Keitarou Saiki and Kiyoshi Konishi and Tomoharu Gomi and Tamao Nishihara and Masanosuke Yoshikawa},
  journal={Microbiology and immunology},
  year={2001},
  volume={45 6},
  pages={
          497-506
        }
}
Cytolethal distending toxin (CDT) has been found in various pathogenic bacterial species and causes a cell distending and a G2 arrest against eukaryotic cells. All the cdtABC genes, which encode CDT, are known to be required for the CDT activities although the CDT holotoxin structure has not been elucidated. We cloned the cdtABC genes of Actinobacillus actinomycetemcomitans and constructed an Escherichia coli expression system for them. We found that crude extracts from six deletion mutants… CONTINUE READING
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