Reconstitution and Characterization of Budding Yeast-Tubulin Complex

  title={Reconstitution and Characterization of Budding Yeast-Tubulin Complex},
  author={Dani B N Vinh and Joshua W. Kern and William O. Hancock and Jonathon Howard and Trisha N. Davis},
Nucleation of microtubules is central to assembly of the mitotic spindle, which is required for each cell division. -Tubulin is a universal component essential for microtubule nucleation from centrosomes. To elucidate the mechanism of microtubule nucleation in budding yeast we reconstituted and characterized the yeast -tubulin complex (Tub4p complex) produced in insect cells. The recombinant complex has the same sedimentation coefficient (11.6 S) as the native complex in yeast cell extracts and… CONTINUE READING


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Handbook of Biochemistry, Selected Data for Molecular Biology, Cleveland, OH: The Chemical Rubber Co

  • H. A. Sober
  • 1968
Highly Influential
2 Excerpts

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