Reconstituting SNARE-mediated membrane fusion at the single liposome level.

Abstract

Successful reconstitutions of SNARE-mediated intracellular membrane fusion have been achieved in bulk fusion assays since 1998 and in single liposome fusion assays since 2004. Especially in neuronal presynaptic SNARE-mediated exocytosis, fusion is controlled by numerous accessory proteins, of which some functions have also been reconstituted in vitro. The… (More)
DOI: 10.1016/bs.mcb.2015.02.005

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