Reconstituted aquaporin 1 water channels transport CO2 across membranes.

  title={Reconstituted aquaporin 1 water channels transport CO2 across membranes.},
  author={G V Ramesh Prasad and Larry A Coury and Frances M Finn and Mark Lawrence Zeidel},
  journal={The Journal of biological chemistry},
  volume={273 50},
Biological membranes provide selective barriers to a number of molecules and gases. However, the factors that affect permeability to gases remain unclear because of the difficulty of accurately measuring gas movements. To determine the roles of lipid composition and the aquaporin 1 (AQP1) water channel in altering CO2 flux across membranes, we developed a fluorometric assay to measure CO2 entry into vesicles. Maximal CO2 flux was approximately 1000-fold above control values with 0.5 mg/ml… CONTINUE READING