Reconstituted Skin from Murine Embryonic Stem Cells

  title={Reconstituted Skin from Murine Embryonic Stem Cells},
  author={Christelle Coraux and Caroline Hilmi and Matthieu Rouleau and Anne Spadafora and Jocelyne Hinnrasky and J -P Ortonne and Christian Dani and Daniel Aberdam},
  journal={Current Biology},
Embryonic stem (ES) cell lines can be expanded indefinitely in culture while maintaining their potential to differentiate into any cell type. During embryonic development, the skin forms as a result of reciprocal interactions between mesoderm and ectoderm. Here, we report the in vitro differentiation and enrichment of keratinocytes from murine ES cells seeded on extracellular matrix (ECM) in the presence of Bone Morphogenic Protein-4 (BMP-4) or ascorbate. The enriched preparation of… CONTINUE READING