Recombineering: using drug cassettes to knock out genes in vivo.

  title={Recombineering: using drug cassettes to knock out genes in vivo.},
  author={James A. Sawitzke and Lynn C. Thomason and Mikhail G. Bubunenko and Xintian Li and Nina Costantino and Donald L. Court},
  journal={Methods in enzymology},
A 'gene knockout' or 'knockout' is a mutation that inactivates a gene function. These mutations are very useful for classical genetic studies as well as for modern techniques including functional genomics. In the past, knockouts of bacterial genes were often made by transposon mutagenesis. In this case, laborious screens are required to find a knockout in the gene of interest. Knockouts of other organisms have traditionally been made by first using in vitro genetic engineering to modify genes… CONTINUE READING


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