Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination

  title={Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination},
  author={L. Thomason and D. Court and M. Bubunenko and N. Costantino and Helen Wilson and S. Datta and Amos B. Oppenheim},
  journal={Current Protocols in Molecular Biology},
  • L. Thomason, D. Court, +4 authors Amos B. Oppenheim
  • Published 2007
  • Biology, Medicine
  • Current Protocols in Molecular Biology
  • The bacterial chromosome and plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage‐encoded recombination functions efficiently to recombine sequences with homologies as short as 35 to 40 bases. This recombineering allows DNA sequences to be inserted or deleted without regard to location of restriction sites. This unit first describes preparation of electrocompetent cells expressing… CONTINUE READING
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