Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination

@article{Thomason2007RecombineeringGE,
  title={Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination},
  author={L. Thomason and D. Court and Mikail Bubunenko and N. Costantino and H. Wilson and S. Datta and A. Oppenheim},
  journal={Current Protocols in Molecular Biology},
  year={2007},
  volume={78}
}
The bacterial chromosome and plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage‐encoded recombination functions efficiently to recombine sequences with homologies as short as 35 to 40 bases. This recombineering allows DNA sequences to be inserted or deleted without regard to location of restriction sites. This unit first describes preparation of electrocompetent cells expressing… Expand
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