Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination

@article{Thomason2007RecombineeringGE,
  title={Recombineering: Genetic Engineering in Bacteria Using Homologous Recombination},
  author={L. Thomason and D. Court and M. Bubunenko and N. Costantino and Helen Wilson and S. Datta and Amos B. Oppenheim},
  journal={Current Protocols in Molecular Biology},
  year={2007},
  volume={78}
}
  • L. Thomason, D. Court, +4 authors Amos B. Oppenheim
  • Published 2007
  • Biology, Medicine
  • Current Protocols in Molecular Biology
    • The bacterial chromosome and plasmids can be engineered in vivo by homologous recombination using PCR products and synthetic oligonucleotides as substrates. This is possible because bacteriophage‐encoded recombination functions efficiently to recombine sequences with homologies as short as 35 to 40 bases. This recombineering allows DNA sequences to be inserted or deleted without regard to location of restriction sites. This unit first describes preparation of electrocompetent cells expressing… CONTINUE READING
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    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 36 REFERENCES
    One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
    • 11,721
    • PDF
    An efficient recombination system for chromosome engineering in Escherichia coli.
    • 1,560
    • PDF
    Genetic engineering using homologous recombination.
    • 433
    A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.
    • 1,173
    • PDF
    Simple and highly efficient BAC recombineering using galK selection
    • 1,150
    • PDF
    Recombineering: a powerful new tool for mouse functional genomics
    • 751
    • PDF
    A set of recombineering plasmids for gram-negative bacteria.
    • 362
    • PDF
    DNA cloning by homologous recombination in Escherichia coli
    • 379
    • PDF
    High efficiency mutagenesis, repair, and engineering of chromosomal DNA using single-stranded oligonucleotides
    • 483
    • PDF
    PCR-mediated gene replacement in Escherichia coli.
    • 273