Recombination of uracil-containing lambda bacteriophages.

@article{Hays1981RecombinationOU,
  title={Recombination of uracil-containing lambda bacteriophages.},
  author={John B. Hays and Bruce K. Duncan and Sigrun Boehmer},
  journal={Journal of bacteriology},
  year={1981},
  volume={145 1},
  pages={306-20}
}
Controlled incorporation of uracil into the deoxyribonucleic acid (DNA) of lambda bacteriophages was achieved by growth on dut ung thy mutants of Escherichia coli. The frequency of substitution of uracil for thymine, estimated by alkaline sucrose sedimentation of phage DNA treated in vitro with uracil DNA glycosylase, ranged from 0.17 to 1.9%. The… CONTINUE READING