Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences.

@article{Lin1985RecombinationIM,
  title={Recombination in mouse L cells between DNA introduced into cells and homologous chromosomal sequences.},
  author={Feng L. Lin and Karen Sperle and Nat L. Sternberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1985},
  volume={82 5},
  pages={1391-5}
}
In this paper, we show that DNA added to mouse L cells by the calcium phosphate method can be inserted into the genome of those cells by homologous recombination. The insertion event is detected because it reconstructs a functional thymidine kinase (tk) gene from two defective genes that share 320 base pairs of homology. One of the genes is missing its 5' portion (tk delta 5') and is in the cell's chromosome, and the other is missing its 3' portion (tk delta 3') and is in the introduced DNA… CONTINUE READING

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