Recombination can lead to spurious results in retroviral transduction with dually fluorescent reporter genes.

Abstract

Fluorescent proteins are routinely employed as reporters in retroviral vectors. Here, we demonstrate that transduction with retroviral vectors carrying a tandem-dimer Tomato (TdTom) reporter produces two distinct fluorescent cell populations following template jumping due to a single nucleotide polymorphism between the first and second Tomato genes… (More)
DOI: 10.1128/JVI.02524-13

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