Recombination between two TnA transposon sequences oriented as inverse repeats is found less frequently than between direct repeats

@article{Chiang1982RecombinationBT,
  title={Recombination between two TnA transposon sequences oriented as inverse repeats is found less frequently than between direct repeats},
  author={Shu-Jen Chiang and Royston C. Clowes},
  journal={Molecular and General Genetics MGG},
  year={1982},
  volume={185},
  pages={169-175}
}
Inverse repeats of the transposon Tn2660 in either a ColEl or an R6K replicon, with or without inversions of the parental DNA sequences between the repeats, show no detectable (<2%) evidence of recombination between the repeats after 60 generations of growth in either recA or recA + hosts. In contrast, attempts made to construct plasmids which carry two direct repeats by in vitro cleavage and ligation in a recA host were unsuccessful, although homologous plasmids with inverse repeats could be… CONTINUE READING