Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts.

@article{He2007RecombinationAE,
  title={Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts.},
  author={Dong-mei He and Kai-xian Qian and Gui-fang Shen and Zhifang Zhang and Yi-n{\"u} Li and Zhong-Liang Su and Hong-bo Shao},
  journal={Colloids and surfaces. B, Biointerfaces},
  year={2007},
  volume={55 1},
  pages={
          26-30
        }
}
The expression of classical swine fever virus (CSFV) structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after… CONTINUE READING
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