Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

@article{Buchmeier1993RecombinationdeficientMO,
  title={Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages},
  author={Nancy Buchmeier and Craig J. Lipps and M So and Fred Heffron},
  journal={Molecular Microbiology},
  year={1993},
  volume={7}
}
Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative… 
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These data demonstrate that an equilibrium between the intracellular concentrations of RecA and CheW is necessary for swarming in S. enterica, and are the first to point out that the SOS response plays a critical role in the prevention of DNA damage by abolishing bacterialSwarming in the presence of a genotoxic compound.
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