Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages

@article{Buchmeier1993RecombinationdeficientMO,
  title={Recombination‐deficient mutants of Salmonella typhimurium are avirulent and sensitive to the oxidative burst of macrophages},
  author={N. Buchmeier and C. Lipps and M. Y. So and F. Heffron},
  journal={Molecular Microbiology},
  year={1993},
  volume={7}
}
  • N. Buchmeier, C. Lipps, +1 author F. Heffron
  • Published 1993
  • Biology, Medicine
  • Molecular Microbiology
  • Mutations in the genes recA and recBC were constructed in the virulent Salmonella typhimurium strain 14028s. Both the recA and recBC mutants were attenuated in mice. The mutants were also sensitive to killing by macrophages in vitro. The recombination mutants were no longer macrophage sensitive in a variant line of J774 macrophage‐like cells that fail to generate superoxide. This suggests that repair of DNA damage by Salmonella is necessary for full virulence in vivo and that the oxidative… CONTINUE READING
    185 Citations

    Topics from this paper

    Role of the RecBCD recombination pathway in Salmonella virulence.
    • 50
    • PDF
    The role of DNA base excision repair in the pathogenesis of Salmonella enterica serovar Typhimurium
    • 67
    Effect of mutS and recDMutations on Salmonella Virulence
    • 24
    • PDF
    Molecular Characterization of the Prototrophic Salmonella Mutants Defective for Intraepithelial Replication
    • 4
    • PDF
    DNA repair is more important than catalase for Salmonella virulence in mice.
    • 149
    • PDF

    References

    SHOWING 1-10 OF 28 REFERENCES
    Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent.
    • 1,042
    • PDF
    Intracellular survival of wild-type Salmonella typhimurium and macrophage-sensitive mutants in diverse populations of macrophages.
    • 217
    • PDF
    Cloning and molecular characterization of genes whose products allow Salmonella typhimurium to penetrate tissue culture cells.
    • J. E. Galán, R. Curtiss
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1989
    • 897
    • PDF
    INFECTION-IMMUNITY IN EXPERIMENTAL SALMONELLOSIS
    • 146
    • PDF
    Penetration of human intestinal epithelial cells by Salmonella: molecular cloning and expression of Salmonella typhi invasion determinants in Escherichia coli.
    • 143
    • PDF