Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.

  title={Recombinase polymerase amplification assay for rapid detection of Francisella tularensis.},
  author={Milena Euler and Yongjie Wang and Peter Otto and Herbert Tomaso and Raquel Escudero and Pedro Anda and Frank T. Hufert and Manfred Weidmann},
  journal={Journal of clinical microbiology},
  volume={50 7},
Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time… CONTINUE READING
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