Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection.

@article{Kaneko2005RecombinasemediatedMT,
  title={Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection.},
  author={Takehito Kaneko and Stefan Moisyadi and Ryota Suganuma and Barbara B. Hohn and Ryuzo Yanagimachi and Pawel Pelczar},
  journal={Theriogenology},
  year={2005},
  volume={64 8},
  pages={
          1704-15
        }
}
The low efficiency of current microinjection-based animal transgenesis techniques is largely the result of poor embryo survival. We have developed a new, bacterial recombinase-based transgenesis method. Intracytoplasmic sperm injection (ICSI) of single stranded DNA (ssDNA) complexed with E. coli recombinase RecA into mouse metaphaseII (MII) arrested oocytes resulted in RecA-dependent transgenesis. This approach offers significant advantages over pronuclear microinjection and previous ICSI-based… CONTINUE READING
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