Recombinase-mediated cassette exchange provides a versatile platform for gene targeting: knockout of miR-31b.


A series of vectors has been designed to enhance the versatility of targeted homologous recombination. Recombinase-mediated cassette exchange permits sequential targeting at any locus and improves flexibility in making user-defined mutations. Application of RMCE to delete an intronic microRNA gene is described. 
DOI: 10.1534/genetics.109.105213


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