Recombinant truncated AniA of pathogenic Neisseria elicits a non-native immune response and functional blocking antibodies.

@article{Shewell2013RecombinantTA,
  title={Recombinant truncated AniA of pathogenic Neisseria elicits a non-native immune response and functional blocking antibodies.},
  author={Lucy K. Shewell and Shan Chi Ku and Benjamin L Schulz and Freda E-C Jen and Tsitsi D. Mubaiwa and Margaret R. Ketterer and Michael A. Apicella and Michael P Jennings},
  journal={Biochemical and biophysical research communications},
  year={2013},
  volume={431 2},
  pages={215-20}
}
AniA of the pathogenic Neisseria is glycosylated in its C-terminal repeat region by the pilin glycosylation (pgl) pathway. AniA appears to be unique among bacterial nitrite reductases as it contains an N-terminal extension that includes a lipid modification site as well as a C-terminal extension that is glycosylated. Immunising with various glycoforms of the AniA protein demonstrated a strong humoral immune response to the basal monosaccharide. In addition, when animals were immunised with a… CONTINUE READING