Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV.

@article{Lilley1994RecombinantSA,
  title={Recombinant single-chain antibody peptide conjugates expressed in Escherichia coli for the rapid diagnosis of HIV.},
  author={Glenn G. Lilley and Olan Dolezal and Carmel J. Hillyard and C{\'e}cile Bernard and Peter J. Hudson},
  journal={Journal of immunological methods},
  year={1994},
  volume={171 2},
  pages={211-26}
}
Recombinant single chain Fv (scFv) antibody fragments can form the basis of a rapid, whole-blood diagnostic assay. The scFv described in this study is derived from a monoclonal antibody which has a high affinity for glycophorin A, an abundant glycoprotein on the human red blood cell membrane surface. The prototype reagent built around the scFv was designed to detect, in whole blood samples, the presence of antibodies that have arisen through infection with a foreign organism such as human… CONTINUE READING