Recombinant protein secretion in Escherichia coli.

  title={Recombinant protein secretion in Escherichia coli.},
  author={Filipe Jos{\'e} Mergulh{\~a}o and David K. Summers and Gabriel Ant{\'o}nio Amaro Monteiro},
  journal={Biotechnology advances},
  volume={23 3},
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a reduced process cost. This review presents several strategies that can be used for recombinant protein… CONTINUE READING
Highly Influential
This paper has highly influenced 17 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
148 Citations
226 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 148 extracted citations


Publications referenced by this paper.
Showing 1-10 of 226 references

Secretion capacity limitations of the Sec pathway in Escherichia coli

  • F Mergulhão, G. Monteiro
  • J Microb Biotechnol
  • 2004
Highly Influential
5 Excerpts

Recombinant human proinsulin: a new approach in gene assembly and protein expression

  • F Mergulhão, G Monteiro, A Kelly, M Taipa, J. Cabral
  • J Microb Biotechnol
  • 2000
Highly Influential
3 Excerpts

Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli

  • FJM Mergulhão, GA Monteiro, JMS Cabral, MA. Taipa
  • J Microb Biotechnol 2004b;14:1–14
  • 2004
Highly Influential
6 Excerpts

Similar Papers

Loading similar papers…