Recombinant protein secretion in Escherichia coli.

@article{Mergulho2005RecombinantPS,
  title={Recombinant protein secretion in Escherichia coli.},
  author={Filipe Jos{\'e} Mergulh{\~a}o and David K. Summers and Gabriel Ant{\'o}nio Amaro Monteiro},
  journal={Biotechnology advances},
  year={2005},
  volume={23 3},
  pages={177-202}
}
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of soluble and biologically active proteins at a reduced process cost. This review presents several strategies that can be used for recombinant protein… CONTINUE READING
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Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli

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