Recombinant protein expression in Pichia pastoris.

  title={Recombinant protein expression in Pichia pastoris.},
  author={James M. Cregg and Joan Lin Cereghino and Jianping Shi and David R. Higgins},
  journal={Molecular biotechnology},
  volume={16 1},
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool. What began more than 20 years ago as a program to convert abundant methanol to a protein source for animal feed has been developed into what is today two important biological tools: a model eukaryote used in cell biology research… CONTINUE READING
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