Recombinant protein expression in Pichia pastoris

@article{Cregg2000RecombinantPE,
  title={Recombinant protein expression in Pichia pastoris},
  author={James Michael Cregg and Joan Lin Cereghino and Jianying Shi and David R. Higgins},
  journal={Molecular Biotechnology},
  year={2000},
  volume={16},
  pages={23-52}
}
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular biology for the generation of recombinant protein. P. pastoris has demonstrated its most powerful success as a large-scale (fermentation) recombinant protein production tool. What began more than 20 years ago as a program to convert abundant methanol to a protein source for animal feed has been developed into what is today two important biological tools: a model eukaryote used in cell biology research… 

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This chapter reviews common and new "Pichia tools" and their specific features, special focus is given to expression strains, such as different methanol utilization, protease-deficient or glycoengineered strains, combined with application highlights.

Genome sequence of the recombinant protein production host Pichia pastoris

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Methods for efficient high-throughput screening of protein expression in recombinant Pichia pastoris strains.

Methods to detect and analyze protein expression, developed in a 96-well format for high-throughput screening of recombinant P. pastoris strains are described.

Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris.

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Pichia pastoris: A notable heterologous expression system for the production of foreign proteins—Vaccines

Yeast expression systems are expanding the role of yeast in the process of understanding and engineering eukaryotic proteins, and Pichia pastoris has become a highly popular expression host for the production of a wide variety of intracellular and extracellular recombinant proteins of interest.

Production of Humanlike Recombinant Proteins in Pichia pastoris

Renewed interest in yeast and fungal expression systems in general and the P. pastoris expression system in particular has been spurred by a growing demand for scalable and costeffective humanlike therapeutic protein manufacturing systems.

Heterologous protein production using the Pichia pastoris expression system

The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins and the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed.
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