Recombinant prion protein induces rapid polarization and development of synapses in embryonic rat hippocampal neurons in vitro

@article{Kanaani2005RecombinantPP,
  title={Recombinant prion protein induces rapid polarization and development of synapses in embryonic rat hippocampal neurons in vitro},
  author={J. Kanaani and S. Prusiner and J. Diacovo and S. Baekkeskov and G. Legname},
  journal={Journal of Neurochemistry},
  year={2005},
  volume={95}
}
  • J. Kanaani, S. Prusiner, +2 authors G. Legname
  • Published 2005
  • Biology, Medicine
  • Journal of Neurochemistry
  • While a β‐sheet‐rich form of the prion protein (PrPSc) causes neurodegeneration, the biological activity of its precursor, the cellular prion protein (PrPC), has been elusive. We have studied the effect of purified recombinant prion protein (recPrP) on rat fetal hippocampal neurons in culture. Overnight exposure to Syrian hamster or mouse recPrP, folded into an α‐helical‐rich conformation similar to that of PrPC, resulted in a 1.9‐fold increase in neurons with a differentiated axon, a 13.5‐fold… CONTINUE READING
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