Recombinant prion protein induces rapid polarization and development of synapses in embryonic rat hippocampal neurons in vitro
@article{Kanaani2005RecombinantPP, title={Recombinant prion protein induces rapid polarization and development of synapses in embryonic rat hippocampal neurons in vitro}, author={J. Kanaani and S. Prusiner and J. Diacovo and S. Baekkeskov and G. Legname}, journal={Journal of Neurochemistry}, year={2005}, volume={95} }
While a β‐sheet‐rich form of the prion protein (PrPSc) causes neurodegeneration, the biological activity of its precursor, the cellular prion protein (PrPC), has been elusive. We have studied the effect of purified recombinant prion protein (recPrP) on rat fetal hippocampal neurons in culture. Overnight exposure to Syrian hamster or mouse recPrP, folded into an α‐helical‐rich conformation similar to that of PrPC, resulted in a 1.9‐fold increase in neurons with a differentiated axon, a 13.5‐fold… CONTINUE READING
Topics from this paper
Paper Mentions
Blog Post
169 Citations
Deletion of the amino‐terminal domain of the prion protein does not impair prion protein–dependent neuronal differentiation and neuritogenesis
- Biology, Medicine
- Journal of neuroscience research
- 2009
- 7
- Highly Influenced
Cellular Prion Protein Signaling in Serotonergic Neuronal Cells
- Biology, Medicine
- Annals of the New York Academy of Sciences
- 2007
- 43
Expression and knockdown of cellular prion protein (PrPC) in differentiating mouse embryonic stem cells.
- Biology, Medicine
- Differentiation; research in biological diversity
- 2011
- 46
- Highly Influenced
PrPC Controls via Protein Kinase A the Direction of Synaptic Plasticity in the Immature Hippocampus
- Biology, Medicine
- The Journal of Neuroscience
- 2013
- 37
- PDF
Characterization of prion protein function by focal neurite stimulation
- Biology, Medicine
- Journal of Cell Science
- 2016
- 19
- Highly Influenced
- PDF
Developmental expression of the cellular prion protein (PrPC) in bovine embryos
- Biology, Medicine
- Molecular reproduction and development
- 2012
- 22
Developmental changes in cellular prion protein in primate visual cortex
- Biology, Medicine
- The Journal of comparative neurology
- 2007
- 4
Prion protein (PrPc) positively regulates neural precursor proliferation during developmental and adult mammalian neurogenesis.
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 2006
- 252
- PDF
Neurodevelopmental expression and localization of the cellular prion protein in the central nervous system of the mouse
- Biology, Medicine
- The Journal of comparative neurology
- 2010
- 24
References
SHOWING 1-10 OF 49 REFERENCES
Prion protein as trans-interacting partner for neurons is involved in neurite outgrowth and neuronal survival
- Biology, Medicine
- Molecular and Cellular Neuroscience
- 2003
- 188
- Highly Influential
Mice devoid of prion protein have cognitive deficits that are rescued by reconstitution of PrP in neurons
- Biology, Medicine
- Neurobiology of Disease
- 2005
- 166
Developmental expression of the cellular prion protein in elongating axons
- Biology, Medicine
- The European journal of neuroscience
- 2002
- 82
Normal neurogenesis and scrapie pathogenesis in neural grafts lacking the prion protein homologue Doppel
- Medicine, Biology
- EMBO reports
- 2001
- 65
- PDF
The human 37-kDa laminin receptor precursor interacts with the prion protein in eukaryotic cells
- Medicine
- Nature Medicine
- 1997
- 437
Prion and doppel proteins bind to granule cells of the cerebellum
- Biology, Medicine
- Proceedings of the National Academy of Sciences of the United States of America
- 2002
- 30
- PDF
Prion protein PrPc interacts with molecular chaperones of the Hsp60 family.
- Biology, Medicine
- Journal of virology
- 1996
- 149
- PDF
Cellular prion protein binds laminin and mediates neuritogenesis.
- Biology, Medicine
- Brain research. Molecular brain research
- 2000
- 315
A transmembrane form of the prion protein in neurodegenerative disease.
- Biology, Medicine
- Science
- 1998
- 713
- PDF