Recombinant lymphocytic choriomeningitis virus expressing vesicular stomatitis virus glycoprotein.

@article{Pinschewer2003RecombinantLC,
  title={Recombinant lymphocytic choriomeningitis virus expressing vesicular stomatitis virus glycoprotein.},
  author={Daniel D Pinschewer and Mar P{\'e}rez and Ana B. Sanchez and Juan C de la Torre},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2003},
  volume={100 13},
  pages={7895-900}
}
A recombinant S segment RNA (Sr) of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) where the glycoprotein of vesicular stomatitis virus (VSVG) was substituted for the glycoprotein of LCMV (LCMV-GP) was produced intracellularly from cDNA under the control of a polymerase I promoter. Coexpression of the LCMV proteins NP and L allowed expression of VSVG from Sr. Infection of transfected cells with WT LCMV (LCMVwt) resulted in reassortment of the L segment of LCMVwt with the Sr… CONTINUE READING