Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite.

  title={Recombinant luminescent bacteria for measuring bioavailable arsenite and antimonite.},
  author={Susanna Tauriainen and Matti Karp and W Chang and Marko Virta},
  journal={Applied and environmental microbiology},
  volume={63 11},
Luminescent bacterial strains for the measurement of bioavailable arsenite and antimony were constructed. The expression of firefly luciferase was controlled by the regulatory unit of the ars operon of Staphylococcus aureus plasmid pI258 in recombinant plasmid pTOO21, with S. aureus RN4220, Bacillus subtilis BR151, and Escherichia coli MC1061 as host strains. Strain RN4220(pTOO21) was found to be the most sensitive for metal detection responding to arsenite, antimonite, and cadmium, the lowest… CONTINUE READING

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